Cryo-electron microscopy (cryoEM) is an important tool in biological research for its ability to visualize nanometer-scale structures in 3-dimensions. Researchers have now developed an algorithm to allow even higher-resolution images and models to be constructed from cryoEM images.
A Japanese-UK research team has now demonstrated that cryoEM image analysis may be exploited to obtain structural information of sufficient resolution to reveal the absolute three-dimensional (3D) configuration of a designed DNA nanostructure. With their technique they have obtained structural information at sufficient resolution to visualize the DNA helix and reveal the absolute stereochemistry of a self-assembled DNA tetrahedron.
